FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_53.351000000393ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_53.351000000393ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486816
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACG7650.15714356142772629No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCG7420.15241898376388616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG1100.021.81870530
GTGCGAC1252.0008883E-1118.00043129
CTCCGCA500.001109560317.998589
AAGACGG601.8640133E-417.4986215
ACTCCGC601.8640133E-417.4986218
TAGGTAG805.1646402E-616.873675
TAAGGGT908.5644933E-716.6653544
TAGCTTG550.002100407816.3623475
GTTCCAG755.5942684E-516.0053161
GTATTAG1351.0950316E-915.5607241
GGTGCGA1451.8189894E-1015.51761328
ACAGTGT706.0316076E-414.9988186
AGGTGCG1554.947651E-1014.51647827
GGTGTAC750.001016281414.00033628
TCTAGGG750.001017071113.9988973
GAACGTT650.00638240913.8450636
CCAACGG902.6441924E-413.33502220
ATTAGGT902.648796E-413.3322823
CAGATGA902.648796E-413.33228213
TGCTAGA800.001655002213.1239672
Produced by FastQC (version 0.11.2)