FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_52.3510000003936a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_52.3510000003936a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924143
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG2450.014.699261
GTGCGAC952.633003E-514.21111629
CGCCGCG650.006380758713.84747824
TCGGGTG650.006383046513.84672825
ACTATGC2450.013.468498
CTCTTAG1252.5627238E-512.00439551
TGCGACG1006.4446125E-412.00049830
TAGACAG1006.4504775E-411.9991995
GTATTAA2950.011.6991991
GAACCGC900.004008065411.6658896
CTTAGAC1303.8442085E-511.5376913
ACACAGT1703.7612335E-711.4698246
GGTGCGA1059.704269E-411.42904628
TATACTG2853.6379788E-1211.0518945
GTCCTAT1253.4084026E-410.8039561
AGACAGC1253.4220796E-410.7992796
TCTATAC2504.5292836E-1010.7992793
TAGGGTC1000.00877923710.4992995
ATGCCGA1150.00207523910.43408611
CTGGTCA1304.915392E-410.3839229
Produced by FastQC (version 0.11.2)