FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_51.35100000039327.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_51.35100000039327.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541209
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC3800.015.7887733
ACTGTGC1801.8189894E-1214.9993358
GGTACGC800.001657407113.1244188
CCTAACC1006.4535736E-411.9994683
AGGAGTC1406.3209354E-611.7851927
GGACTGC1551.5449514E-611.612396
CTATGCA3900.011.537959
GTATTAG4650.011.2927521
TGGGGGG1202.3460598E-411.2495015
TAGGTAG1901.3255885E-711.0521415
ACTATGC3400.011.0289228
TCTAAGC1501.3607321E-510.9995133
CCTAATA4800.010.6245292
TAATACT6750.010.4439814
CCCTAAA2105.234433E-710.0021521
CCAGGAC1200.00295592039.9995573
TATTAGG4800.09.9995572
CCTATAC3101.8553692E-109.67699053
TAATACC4500.09.6662384
AGCCGCA1250.00414312359.59957416
Produced by FastQC (version 0.11.2)