FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_50.351000000392e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_50.351000000392e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898484
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGC601.8650254E-417.50009723
GCCGCGT750.001017170714.00085825
CCCTAGA902.6520604E-413.3326652
GTCCTAG800.001650304213.1309191
CGCCGCG800.001655154213.12580324
CTAATAC3350.012.9844253
GTCTAGG850.002602677812.3585131
GACCGTC1354.207235E-612.221617
CCTTAAG1006.4221676E-412.0054111
CCGGATC1006.443239E-412.00073425
CTGTGAG1006.4492703E-411.9993999
TCTGGAC1252.5734884E-511.9993983
CTTATAC2800.011.7851243
AGGCATG900.004007419611.6660826
ACCTAAT2206.220944E-1011.5961371
TAGCCTG1059.71126E-411.4279995
ATTATAC3700.011.3507833
ACACATC1202.344114E-411.2494368
GGACCAT1602.2602871E-611.2494366
CGCGTCG950.005992149511.05330827
Produced by FastQC (version 0.11.2)