FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_49.351000000392bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_49.351000000392bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851049
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG3500.015.8610431
ACCGTGC1054.274225E-614.2853688
CCGTGCA902.6538598E-413.333019
TAGGTAG2550.012.9408625
TATTAGG3600.012.9163532
TTAGGCA1702.8310751E-812.352644
GGTAGTC2351.2732926E-1112.1273657
TAGTCAG2553.6379788E-1211.76442059
GTGTTAC2351.5279511E-1011.4921871
CCGCTCA1355.6529425E-511.1108429
TAGACAG3600.010.8330715
TACCTAG1408.18139E-510.71402555
GTCTTAG2156.429582E-810.467691
TATACCG2453.7289283E-910.407915
CAGGCCC1304.918463E-410.3843639
GTCCTAA1901.574037E-610.2656811
TACACCG2053.7758036E-710.2436545
CGGTCGA1356.9609616E-49.99975710
CCTATAC2701.7262209E-99.9997573
ATAGGAG2905.693437E-109.8273473
Produced by FastQC (version 0.11.2)