FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_48.35100000039278.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_48.35100000039278.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280479
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGGCA303.4828096E-529.9983989
GTATAGA504.576009E-521.0063691
TTATACT504.588608E-520.9988774
CCCTAAT400.00648714518.7556861
TAGGTAG651.6394455E-518.4605525
GTATTAG854.6717832E-717.652411
GGTAAGA601.8550309E-417.5053061
TATACTG805.1476836E-616.8740985
CCTAATA908.530478E-716.6657752
GTATAGG550.002092072716.3685991
CAGATGA653.4264394E-416.15298313
TATACGG653.4264394E-416.1529835
TAGTCAG653.4264394E-416.1529839
TTAGGTA755.5991575E-515.99914554
CTAATAC951.4999368E-615.788633
GGATATG706.0028787E-415.0045491
GTATAAA1054.2287757E-614.2900471
GACATAG750.001013621214.00174730
TCTATAC750.00101498913.9992523
GTATAAT650.006357406313.8503521
Produced by FastQC (version 0.11.2)