FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_47.35100000039235.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_47.35100000039235.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553986
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAC559.548722E-519.0959071
GCGTCAG908.563493E-716.6665174
TATTAGG1206.8497684E-814.9998662
CAGCGTC952.6305022E-514.2103982
GTATTAG1504.8567017E-914.0036661
CAGGATG650.006380927313.8460289
CGTCAGA1251.7718594E-613.1998825
AGTGGGT800.001654635413.1248818
TAGGTAG1056.738064E-512.8570275
TTAGGTA1302.751347E-612.6921924
ATTAGGT1201.6896713E-512.4998883
CCTTACA1006.4401695E-411.9998912
GGACAGT1151.575705E-411.7390246
TACTCCA900.004002875611.6665627
TACACTG900.004002875611.6665625
AGCGTCA1303.8354698E-511.5383573
TCCTATA1303.8354698E-511.5383572
CTCCAAG1059.6756674E-411.4315641
GTGTATG1059.6756674E-411.4315641
TCCAAGA1501.355846E-510.9999012
Produced by FastQC (version 0.11.2)