FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_46.351000000391f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_46.351000000391f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378636
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGT300.001226577224.9946064
TCCTAGG559.5735944E-519.086792
CCTAGGT400.006510722518.7459563
ATTAGGT1051.3236786E-817.139163
GTTATAC653.4346487E-416.1503623
GTCCTAG600.0037432315.0027081
GTATTAG1251.1233897E-714.4025991
CTTATAC1156.9250564E-714.344733
CTAAAGT650.006384744313.8431674
TAGGTAG902.6494308E-413.3304575
CCACTAT800.001650590613.127371
CTATTAA1056.7151705E-512.8594651
TTATCCT2001.2732926E-1012.747254
TAATACT2401.8189894E-1212.4973034
TAGAACT850.00261090412.3502774
TAAGCTT850.00261090412.3502775
GTATAGA1006.421624E-412.0021661
AGGTAGT1006.4430566E-411.9974126
AATACCG1006.4430566E-411.9974125
AAAGCTA1006.4430566E-411.9974125
Produced by FastQC (version 0.11.2)