FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_45.351000000391bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_45.351000000391bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258603
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACT350.003002062421.4259474
TACACAC400.006501771518.7477045
TCATATG653.426949E-416.1518672
GTGTTAG706.020058E-414.9981631
CCATTAT1156.8932786E-714.3460681
CTTTATA750.001015142713.9982842
TAGACAG650.006373591713.8444585
TTAGACA650.006373591713.8444584
ACTAGAG650.006373591713.8444583
CATTATG1004.2503903E-513.4983472
GGTATAT800.001651895113.1233921
ATTATGC1101.03806524E-412.2712253
CTAAGCA900.00399640111.6652374
CTAATAC900.00399640111.6652373
TATATGA1059.6763E-411.4271725
TTTTAAA1253.4058723E-410.7986782
GTATATA2104.501453E-810.7129741
TATACTT1551.9404419E-510.6438585
CTATACA1000.00875418410.4987144
ATAATAC1200.00294366349.99877553
Produced by FastQC (version 0.11.2)