FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_44.35100000039179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_44.35100000039179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412798
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG706.067603E-821.4340251
TATTAGG559.559524E-519.091142
CTCTTAG400.006494042518.7547721
CACCTAG400.006503136418.75022728
AGTGCGG400.00650768718.7479558
CTGTGCC400.00650768718.7479559
TAGAGTG500.001108494618.0002175
ACCTAGC500.001108494618.00021729
CTAGCAC550.002098404616.3638343
GTCTTAG600.003742152615.0038181
CGATGGA600.003748192215.00018127
TAGGAGT600.003748192215.0001814
ATGCCGC600.003748192215.0001816
CCTAATA851.6292762E-414.1178182
ATACTAT1701.3096724E-1014.1178186
TACTGCG650.00637639913.8463217
AAGTGCG650.00637639913.8463217
TAATACT1557.705239E-913.548554
AATACTA1557.705239E-913.548555
TAAAGGT1004.2570486E-513.5001644
Produced by FastQC (version 0.11.2)