FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_43.35100000039129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_43.35100000039129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320050
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAATA350.002994582521.4370561
TGTACCT400.006503211318.7486325
GGTAGCC400.006503211318.7486328
CCTAGAA601.861413E-417.4987242
CTAATGG601.861413E-417.4987243
TATAGTA600.00374786214.9989065
ATACACA600.00374786214.9989064
CTTCTAA1156.86774E-714.3535071
GTATATG750.001012094814.0055431
GTATTAG650.006356088413.8516361
TCTTAAG650.006375863713.8451442
TAAACCC650.006375863713.8451444
GAAAGGT650.006375863713.8451444
GTATATA1404.450867E-712.8622341
ATTATAC850.002606588612.3520413
TATTGTA1006.430533E-411.99912552
TACCATG1059.683279E-411.4277377
TTATACC1059.683279E-411.4277374
ATATCCT1355.621966E-511.1103014
GTACCAA950.005963000511.0570091
Produced by FastQC (version 0.11.2)