FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_42.351000000390e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_42.351000000390e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862551
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC755.6212237E-515.999013
TCAGTCC809.752798E-514.9990748
ATAGGGG706.0375285E-414.9990723
AGCCGGA650.006379605713.84770628
GTATATG3300.013.1855891
GTATTAG2001.2732926E-1012.7536471
TATTAGG2255.456968E-1212.6658822
GTCCTGA1006.430614E-412.0034331
TAATACT4000.011.9992584
TAGGTAG1252.5735177E-511.9992585
TGTCCCA1006.4494455E-411.9992585
CGTTCCC900.004002345711.667973528
GTCTTAA1551.5362202E-611.6162251
AGGTAGT2103.7325663E-911.4278646
TAAGACA1459.329153E-611.3786064
CTGTACC1602.2602617E-611.2493064
GTATTAA2301.3023964E-911.0901281
GTACTAT1901.3165845E-711.0557931
CCTCTAG950.00598180311.0557931
AGGGTGT1100.001432793310.9084168
Produced by FastQC (version 0.11.2)