FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_41.351000000390a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_41.351000000390a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715818
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC750.001017591313.9994466
GTATTAG1853.45608E-1113.78805351
CTAATAC1601.2090823E-813.124483
CTTAGAC1151.09202265E-513.0429613
CCTAATA1753.0631782E-912.8566332
AACGTAT954.1878564E-412.63019710
GCCTTAG850.00260454212.3567681
GGACAGT850.002611376812.3524516
TAGACAG1354.203368E-612.2217395
TACTATG1601.7096863E-712.1870177
GTCCTAT1006.426976E-412.0037171
TGGACAG1252.5714951E-511.9995265
TTACAGT1501.0376571E-611.9995254
ATACTAT2650.011.8863236
CTATACC1059.706188E-411.4281184
GGACAGA1059.706188E-411.4281186
CAGATCA1059.7118696E-411.42732059
TCTATAC1459.320658E-611.378863
ATAACAC1859.135147E-811.3509033
CTACACT1355.6426077E-511.1106714
Produced by FastQC (version 0.11.2)