FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_40.3510000003906d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_40.3510000003906d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324904
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCC455.4549763E-420.0000028
GACCATG500.001107894918.07
TATTAGG703.101329E-517.1428572
CATTAGC550.002097280716.3636361
TATAGTG550.002097280716.3636365
TCTGTGC653.427136E-416.1538478
GTATTGG755.6009812E-516.01
TCCTATA1052.4619658E-715.7142862
TTAATCT600.00374620715.04
TGTATGC750.001015187514.03
GCTATAC650.0063730613.8461533
ACAGCTA650.0063730613.8461538
GTACTAG1004.252076E-513.51
AGAAGTG800.001651959613.1250015
TAGTACC850.0026053112.35294154
GTATTAA1006.427057E-412.01
ATAGTGT1006.427057E-412.06
GTATTGA900.003996516611.6666671
TTATAGA1059.6780603E-411.4285724
GTATTAG1202.3342796E-411.251
Produced by FastQC (version 0.11.2)