FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_39.3510000003902a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_39.3510000003902a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596144
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAC200.005146559329.998491
GTCTATG859.192794E-615.8815551
TAGGTAG600.003752355514.9992455
GCTGCAC600.003752355514.9992456
TCTAGGC706.0331245E-414.9992442
GTTAAGC650.00637987913.84661827
TCTATAC1601.2076271E-813.1243393
ATACTGA1855.0386006E-1012.9723216
TAATACT2900.012.9303844
TCTGTCC954.1839428E-412.6309438
GTGTATG954.1839428E-412.6309431
GTGTTAC850.002610702312.3523211
TATACCG1101.0417635E-412.272115
GTATAGG1856.9921953E-912.16155051
TAGACAG1006.443671E-411.9993965
GTATTAG1151.576697E-411.738541
GTAATAC2052.5484042E-911.7067283
CCTATAC900.004004721511.66607953
CGGTGTT1059.702896E-411.4279979
CTACACA1059.702896E-411.4279974
Produced by FastQC (version 0.11.2)