FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_38.35100000038fe8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_38.35100000038fe8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660582
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG755.5975073E-516.0058561
TAAGGGT901.5797172E-514.9998124
GTATAAA1156.9021553E-714.3530781
CCTGAAT954.1836762E-412.631423
GTCTTAT1201.6843343E-512.5045751
TTAGACC850.002610480912.3527854
TATAAAG1754.2200554E-811.9998482
TAAGACT1652.5481677E-711.8180334
ACAGTGC1151.5766376E-411.7389828
AGACAGC900.00400437711.666526
TAGACAG1303.8382175E-511.5383165
CCTTATA1059.70226E-411.4284282
TCAATAC1459.31483E-611.3791673
ATAAGAC1202.341513E-411.2498583
TATAGGT1202.341513E-411.2498584
GTATTAT1755.4324846E-711.1469351
GTATTAA1755.4324846E-711.1469351
GAATAGA1501.3513129E-511.0040251
AAGACAG1501.3569965E-510.9998615
GACAGTG1100.00143143510.9089547
Produced by FastQC (version 0.11.2)