FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_37.35100000038fa5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_37.35100000038fa5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948917
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG850.002612336312.352535
TCTAGGG850.002612336312.352533
GTATAAT4100.010.9792941
GAGTGTT1408.172476E-510.7139287
TATACCG1602.773491E-510.3121565
TTAGACT1756.6072425E-610.2853714
ACTGCTC1356.9548894E-49.9996668
GCTATCG1200.00295386679.99966613
CCTATAC1809.24091E-69.9996663
TATTATA5600.09.9103842
CTGATGT1851.2795374E-59.7294054
ATACCGT1851.2795374E-59.7294056
CAAGGGA1705.4005082E-59.7055594
ATTATAC4350.09.654853
GTCCAGG1409.67626E-49.6460931
TTATACG1409.709015E-49.6425354
TAGACTG1250.00414026679.599685
GTATGTA3306.002665E-119.5486581
TTATCCT2701.8266292E-89.4441294
ATACTGC2701.8266292E-89.4441296
Produced by FastQC (version 0.11.2)