FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_36.35100000038f61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_36.35100000038f61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486563
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGC350.003003929521.4282788
AGGGTCT500.001109074617.9997546
GTGAGCA600.003750120814.9997949
CTAATAC1456.8695044E-712.4136233
TATGACT850.002608795912.3527734
GCATATA1602.2412078E-611.2544721
TAGAGCT1202.3391255E-411.24984554
TAGCACT950.005988802811.052484
ATAACAC1501.35497685E-510.9998493
ACCTGCT1000.00876576510.49985623
CCTGTTC1150.00207128910.434648
CTTCCCA1356.940684E-49.9998639
AGAGAGG1200.0029489769.9998638
AGAGAGC1356.940684E-49.9998636
GTATTTA1250.00411945269.6038161
TTGTACT1250.00413346579.5998694
ATATAGC1250.00413346579.5998696
GTATAAA1300.00569158339.2344391
CTATGGT1300.0057108759.2306434
CTACACC1300.0057108759.2306434
Produced by FastQC (version 0.11.2)