FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_35.35100000038f11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_35.35100000038f11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838730
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG3750.012.4012031
TAATACC4600.011.7386724
GTCTTAG2605.456968E-1211.539581
AGACCTG1355.653738E-511.1106795
TCTTATA5800.010.8616462
TATACTG4750.010.73642355
TTATACT6950.010.5751284
ACCGTGT1000.00878297610.4995918
CTTATAC5350.010.3734273
ACAGCGT1501.6448417E-49.999618
GTACATA4750.09.7904221
TATACTT8100.09.6292545
GTATATA8750.09.6009311
CTAGGAC1250.00414358459.5996263
TTAATAC4750.09.4733143
GTATAGG2403.2045318E-79.375911
GCACTGT3051.4188117E-99.3438986
CTAACAC2251.3475928E-69.332973
TAAACTG3552.7284841E-119.2954135
TAGCCTG2105.667722E-69.2853535
Produced by FastQC (version 0.11.2)