FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_34.35100000038edc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_34.35100000038edc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777198
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG455.4663466E-419.9989284
GCACCGT600.003753802714.9991966
GTTATAC1551.5418718E-611.6122813
AATACTG2751.8189894E-1211.4539325
GTCTAAC1202.33364E-411.2544661
ACAGTGC1602.259094E-611.2493978
GTAGCAC1755.4667544E-711.142263
TTAGGAC950.00599603911.0520394
TAGACTG950.00599603911.0520395
GTTATAA1501.3517012E-511.0043661
GTGTGCA1408.169776E-510.7137129
GTCTAGA1000.00874701310.5041681
GCTACAC1000.008776274510.4994373
AGGAGTG1150.002074374610.4342235
TTAGAGG1150.002074374610.4342233
ATTAGAC1304.912911E-410.3840593
CCTAATA1451.16593204E-410.3442732
GTATTAA2352.1433152E-810.216821
GTGTAAC1200.002942330410.0039691
GTGATCA1200.00295333599.9994649
Produced by FastQC (version 0.11.2)