FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_33.35100000038e99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_33.35100000038e99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287269
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCCT350.003000730621.4285726
GTATTAG701.4378711E-619.2857151
GTATATG957.866038E-817.3684221
GTCTAGA550.002096484216.3636361
TCTATGC550.002096484216.3636363
CTAACTC600.003744799215.03
TTACATC600.003744799215.04
TAATACT952.6215926E-514.2105264
AGACTAT650.006370689713.8461536
TAGACAT650.006370689713.8461535
TAAGAGT800.001651148413.1249994
GTATATA1056.715565E-512.8571431
TCTAGTA850.002604042212.35294152
GTATTAT1252.5578991E-512.01
CTATTAA950.005978077711.0526311
TTAAAAG950.005978077711.0526313
TTAGATA1150.002066612710.4347834
ATATACA1603.1265366E-49.3749994
TTATATA1300.00569828839.2307692
TATATAT1350.0077688828.8888892
Produced by FastQC (version 0.11.2)