FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_32.35100000038e56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_32.35100000038e56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689705
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTT350.003006887221.42619114
ACTCCCG601.86383E-417.50059120
AGGTCTG703.1105366E-517.1421955
CCGCCGT653.431341E-416.15556524
CTCCCGC706.03097E-415.00050721
ACGACCT600.003752859314.999425
TAGACTG851.632486E-414.1171015
CTAATAC1853.45608E-1113.7832513
ATGGACC902.6504043E-413.3328183
CTAAGAC800.001655720213.1244943
TAGCCAG800.001655720213.1244945
ATAGGAC1056.744597E-512.8566473
GAGAGTC1302.7550377E-612.6918177
TAATACT2900.012.4133134
TCCCGCC850.002609771412.35335922
GTATAGA1951.1496013E-912.3152521
TAGGACA1252.571153E-511.9995364
TATACCG1406.3063435E-611.7852595
TTACACA1406.3063435E-611.7852594
ATACGAC1151.5772133E-411.7386763
Produced by FastQC (version 0.11.2)