FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_31.35100000038e12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_31.35100000038e12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603172
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCG500.001111235217.9981849
GTATTAG3850.014.4186281
GCGCTCT1056.759375E-512.8558479
GTATAGA5500.012.5481021
GTCCTAA1901.0399162E-811.84460451
TAATACT8250.011.816994
CCTAGCG1151.5769598E-411.74087630
CCTAATA4750.011.6830322
AGACTGT2103.747118E-911.427426
AGGACCG1059.722652E-411.427425
AGCGCTC1459.347143E-611.3781638
CTAGGAC1202.347403E-411.2488653
GTATAAA7050.011.0661661
TCGCCCG950.0059903311.0546224
CAGCTCG950.006002858711.05151710
TCTACAC3200.010.7801633
ATAATAC7100.010.7735613
TAGACTG1851.1222673E-610.5394775
CGCCCGC1000.0087679210.50188925
AAGACCG2002.6911948E-710.498945
Produced by FastQC (version 0.11.2)