FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_30.35100000038ddd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_30.35100000038ddd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758661
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG3950.015.1911971
ACGGACC1004.2683852E-513.50156126
GCCTCAC1551.1340853E-712.58031220
ACAGCGC1354.2139345E-612.2215518
CCGAGCC1006.4534025E-411.99968216
CGGACCA900.00400506811.668015527
TAGGTAG2701.8189894E-1211.6660265
GGTGCGA1551.5423866E-611.61424628
ACTGGCC1859.170617E-811.3507278
GTCTTAA2659.094947E-1211.3217421
CTCGGTC950.005999931611.05233913
AGGTGCG1501.3586234E-511.00127227
CTAAGCC2207.736162E-910.91035326
TATTAGG4700.010.8504682
TAGGCTG1253.4250264E-410.7994065
ACAGTGC2651.09139364E-1010.7541268
ACTAAGC2104.5603883E-810.71552425
TAGGGGT1408.183401E-510.7136974
GGTAGTC2402.6557245E-910.6244157
TCTAAGC2556.4028427E-1010.5876543
Produced by FastQC (version 0.11.2)