FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_29.35100000038da7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_29.35100000038da7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669840
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGC653.435089E-416.15550623
GCCGCGT901.5790734E-515.00334125
GAGCGCA1106.925793E-613.634099
CCGGCTC850.002608756512.35569125
CGCCGCG1151.5759496E-411.74174424
GCGTCAG1151.58079E-411.7378774
GTATTAG4200.011.4297471
GTCTTAA2103.7362042E-911.4297471
ACGGTGT3700.011.3497998
GTCCTAA1602.2599525E-611.2511581
TCGCCGC1355.6448636E-511.11225323
CTGATCG1100.001435266810.9072729
AGCGCAC1807.894978E-710.83152910
TAGACAG2104.5745765E-810.7131415
TAATACT8250.010.5443294
ACTCCGC1451.16874966E-410.3434138
CTAGGAC1756.6227603E-610.28461553
TCTATAC6500.010.1527623
TACACAG3401.8189894E-1210.1459745
CCTAATA5950.010.0829562
Produced by FastQC (version 0.11.2)