FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_28.35100000038d63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_28.35100000038d63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419964
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCTG859.2106275E-615.881575
CGCCGCG600.003753093815.00084524
TACGGTG2900.013.9648287
GCCGCGT650.00638315313.84742225
ACGGTGT3150.012.856518
TATACGG4500.012.3327265
ATACGGT4200.011.4280076
CTGGGTG1459.339699E-611.3787499
CAGGCCC950.005999984711.0520874
ACTATGC3900.010.76878
CGGTGTT3400.010.5877139
CTCTAGA2156.429036E-810.4671811
CGCGTCG1150.002075509610.43463619
AGGGAGC1150.002076117610.4342696
TAATACT6300.010.2375914
TAGAGTG1952.2024033E-69.9995075
CTAATAC4350.09.9995073
TATACCG2301.728622E-79.7821265
TACACAG2301.728622E-79.7821265
GTATTAG4150.09.7609611
Produced by FastQC (version 0.11.2)