FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_27.35100000038d20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_27.35100000038d20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783397
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCC600.00375579714.99952411
GTATTAG4900.013.7785491
ACCTCGC850.002613859512.3525498
ACTGTCC1601.7150705E-712.1871138
CTGGGGT1151.5802137E-411.7384294
TAGTCAG1703.7670725E-711.4702249
ACTGGTC1703.7670725E-711.4702248
TAGACTG1501.3612975E-510.9993425
TAATACT8600.010.8133074
GTCTATA4700.010.5342381
CCGTCGC1000.00878233510.4996669
CTTACAC2752.1645974E-1010.3630173
CTAATAC5300.010.1880693
CTGTGTG1501.6446583E-49.9996839
TAGCGGG1356.961174E-49.9996829
ACCGTGT1200.00295600189.9996828
TCTAGAC2251.2557211E-79.9994023
GTATAAG5150.09.9050971
CCTATAC2454.134381E-89.7953323
TTTATAC6450.09.7668583
Produced by FastQC (version 0.11.2)