FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_26.35100000038cf8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_26.35100000038cf8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212738
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGG400.00651364518.7485313
TAGGTAG2350.016.5944445
GGTAGTC2200.014.9988247
GTATTAG4100.013.9076571
CCCTGCC1004.2740317E-513.4989429
TAGTCAG2450.013.4683339
CAGCGTG1352.8997056E-713.3322899
TTAGGTA3300.013.1807854
GCCTCAC1151.0930187E-513.04353120
CGTCGTG1507.36909E-813.00166130
ATCACAC2450.012.85878729
ATTAGGT3500.012.8561353
CCGGCTC954.1832024E-412.63319425
CCCTGCA954.1890264E-412.6311092
ACTGATC1456.9032103E-712.412828
CTAGACT1006.4536673E-411.999064
TAATACT6200.011.853914
TATTAGG3300.011.8177432
GAGGCCT1406.3202115E-611.78479217
GGACTGG1151.5796885E-411.738216
Produced by FastQC (version 0.11.2)