FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_25.35100000038cb4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_25.35100000038cb4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583371
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGT400.006508460718.7491428
CGTCCGA400.006508460718.7491426
TAGAGTG752.7926144E-617.9991765
AAGGCGT601.8631203E-417.500719
ATCACAC805.162341E-616.87567529
TAGGTAG805.1665556E-616.8742285
TTAGAGT1002.5694553E-614.9993144
CTAATAC2150.013.952853
GCCTTAG650.006375767813.8478951
CCCTATA650.00638301313.845522
ATTAGGT1106.8986938E-613.635743
TAGTCAG902.649465E-413.3327259
CGTTCCC800.001655275913.12439917
GCTCACG800.001655275913.12439912
ACCTAAT1855.0386006E-1012.9746051
TAATACC1855.0386006E-1012.972384
GGTAGTC1056.741576E-512.8565557
CCTAATA2001.2732926E-1012.7494172
TATACAC2001.2732926E-1012.7494173
TCTAGTA1302.7531896E-612.6917272
Produced by FastQC (version 0.11.2)