FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_24.35100000038c71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_24.35100000038c71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629071
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCTC455.4638553E-419.99941610
CCCCGTG400.00650839218.74945320
TAGACTG500.001109680717.9994775
GGGGGGC500.001109680717.9994778
ACGTGAG703.10958E-517.14235911
GTCCTAA653.425976E-416.1585121
CACTGGG653.4342866E-416.1533767
ACTAGAC706.0328085E-414.9995633
CGCTAGG600.003752132914.99956312
TAAGGAC600.003752132914.9995633
CTAATAC1206.856499E-814.9995633
TCCTAAG952.6321213E-514.2101122
TAGATAG851.6319935E-414.1172365
CTTACAC851.6319935E-414.1172363
CATGTAG750.0010172713.99959310
GTACTAA650.006369613613.8501531
CTAGACT650.006383046513.845754
CTAAGAC650.006383046513.845753
CCTAAGG650.006383046513.845753
AGGGGGG650.006383046513.845757
Produced by FastQC (version 0.11.2)