FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_23.35100000038c3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_23.35100000038c3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583671
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG3250.017.9996035
GGTAGTC3450.016.5208537
TAGTCAG3250.015.6914669
GTATTAG5000.013.8040531
GGCGCCG902.6513054E-413.33430325
TCGCCCG800.001655983313.12595324
AGTCAGA4200.012.4993310
GTAGTCA4250.011.9993558
TACTCGG1901.0440999E-811.84146911
ATTAGGT5450.011.83463
TATTAGG6300.011.6664092
GGTCTCG900.004009834511.6660415
GCGCCGA1059.708425E-411.42940126
CTAATAC5800.011.379063
TACCCGG1859.155883E-811.35181722
GCCGTCT1202.3442795E-411.25046222
CTATGCA4400.011.2493969
TTAGGTA5150.011.0677174
CCGTCTA1100.001432710310.90953823
TAATACT8900.010.6177434
Produced by FastQC (version 0.11.2)