FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_22.35100000038bf9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_22.35100000038bf9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745029
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGAG653.4366344E-416.1525823
GTATTAG1504.8621587E-914.0045441
TAAGACT750.001017957213.9989034
ACAGCCC650.00638941913.8441418
GGTTCGA650.00638941913.8441418
CCTACAC902.651741E-413.3322893
TCCTATA1302.757104E-612.6913152
TAGGTAG1201.6927259E-512.4990225
TTAGTAC1456.891278E-712.4128213
TTATACT2950.012.2024354
GAACTGT1151.5781207E-411.7382116
TAGTACT1551.5420483E-611.6119944
TCCTAAG1059.710145E-411.4276762
ACTGCTC1059.7156054E-411.4269098
GGTATAT2503.6379788E-1111.4036991
AATACAG2259.094947E-1011.3324455
CATATAC3350.011.1931523
CTTAGAC1355.6455494E-511.1102413
CAGATCC950.00599974211.05102359
CATATCC1653.2685584E-610.9082363
Produced by FastQC (version 0.11.2)