FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_21.35100000038bb5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_21.35100000038bb5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125013
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG200.005129930629.99999814
TCCGAGC200.005129930629.99999815
CACGAGA200.005129930629.99999823
GAGACGC200.005129930629.99999826
ACGCTAC200.005129930629.99999829
GAGCCCA200.005129930629.99999818
CGAGCCC200.005129930629.99999817
CGAGACG200.005129930629.99999825
GACGCTA200.005129930629.99999828
AGTGTGG200.005129930629.9999988
GTAAATG350.002991361521.4285721
CATCTCC400.00647883718.74999811
CTCTTAT400.00647883718.7499981
ATACTTT400.00647883718.7499986
CTGCCAG400.00647883718.74999822
GTATAAT400.00647883718.7499981
CTAATAG400.00647883718.7499983
AATGAAA1000.00870385110.52
Produced by FastQC (version 0.11.2)