FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_20.35100000038b72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_20.35100000038b72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338852
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGC200.00513564130.00782629
GTAGAAC452.0356714E-523.3325313
CCAGCGC350.003002430521.4278328
TTACACA455.456905E-419.9993114
GTCCTAT400.00648592618.757661
CTAGGAC400.00650253418.7493553
GTAGTCA500.001108283917.999388
GTATTAT1353.6379788E-1217.7850381
TCTAGGA550.002098012716.3630712
ACTATGC1052.4638393E-715.7137448
GGTTATC600.003740151615.00391127
TATACTG809.722743E-514.9994855
ATACAGC809.722743E-514.9994856
TACTATG901.575535E-514.9994847
CTTATAC600.00374750714.9994833
GGACCCT600.00374750714.9994836
TATACCT600.00374750714.9994834
TAAGCCT600.00374750714.9994834
CTAATAC1002.5614518E-614.9994833
CTATGCA600.00375118914.997279
Produced by FastQC (version 0.11.2)