FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_19.35100000038b3f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_19.35100000038b3f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678283
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACG601.8610849E-417.5064331
TCGCCCG1051.3289537E-817.1435424
TAGGTAG2700.016.1103125
TATTAGG4400.016.021932
TAGGCTA1154.0812665E-815.6513975
GGTAGTC2950.015.253487
TAGTCAG2900.014.482049
GTATTAG6050.014.3854511
GGGGCTC1054.2747743E-614.28500610
CTATGCA4400.013.9765799
TCACACA4000.013.87596530
CACCTAG1955.456968E-1213.847115528
ACCTAGC2009.094947E-1213.50093829
CCTAGCG2250.013.33426130
CCGTCCT902.6541995E-413.332679
CGCATCC1507.381277E-813.00090329
CTAATAC6250.012.9593573
ACTATGC6400.012.8899858
CGCCCGC1404.50671E-712.85803625
ATCACAC4350.012.75950829
Produced by FastQC (version 0.11.2)