FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_18.35100000038afa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_18.35100000038afa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997827
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG2150.013.95482730
GTCCTAA2350.013.407891
ACCTAGC2550.012.35443629
TATACGG4750.012.3151225
ACCTAAT6300.011.907991
GAGAGTC2900.011.8959067
GGTAGTC2800.011.7850757
TGTGCGG1151.5803144E-411.73849410
TATTAGG6050.011.6522612
TAGTCAG2452.5465852E-1111.6320229
CCTAATA7400.011.5534292
CACCTAG2605.456968E-1211.53985828
ATCGTGC1303.8489354E-511.5378358
GTATTAG5350.011.2179951
CCAGCCG1100.001434134710.908513
CCTGCCC1951.8987521E-710.76864610
TAGGTAG3250.010.614815
TAAACCG1704.6838886E-610.5876625
ATCACAC4550.010.55072729
CGATCAC2853.6379788E-1110.52706224
Produced by FastQC (version 0.11.2)