FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_17.35100000038aaa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_17.35100000038aaa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497707
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGCG752.7965289E-618.00054224
GCCGCGT805.1737297E-616.87550725
GGTAGTC3050.014.7535577
TAGTCAG2750.014.7267329
ATCACAC3300.014.54589329
TAGGTAG3250.013.3841255
TTAGGTA4050.013.3328454
CGTGGGC800.001657273713.1245188
CTATGCA3900.013.0764439
CAGCGTG1855.0749804E-1012.9724969
TCACACA3600.012.91705530
TACAGCG2255.456968E-1212.6662027
TATTAGG5400.012.4995412
GTATTAG4700.012.4509241
CCCCGGC850.002612309812.353312523
ACCGTGT850.002613618512.3524888
GACGCAA2001.7371349E-912.00036123
GACCTCC1006.45297E-411.99955918
ATTAGGT5400.011.9440063
CCTAATA6050.011.6524642
Produced by FastQC (version 0.11.2)