FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_16.35100000038a66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_16.35100000038a66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666183
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTGC851.634529E-414.1172526
CCGTCTC800.001657675613.1242389
CACAGGG1056.755075E-512.8567837
CTTAGGC1151.5796276E-411.7388013
ATCACAC4000.011.62572129
GTATAGG3250.011.5416021
CATCACA4450.011.46138528
CCTAATA6200.011.3706492
CTATGCA4650.011.2896679
TCACACA4400.011.250360530
AGGGGTG950.00599977411.0523215
CTAATAC6800.011.0291033
ACTGTGC2053.207424E-810.9749738
ACTATGC5800.010.8614398
TACTATG6550.010.7630577
CAGCGTG1551.9566001E-510.6445449
TTAGGTA4550.010.5491554
AGGGGCG1000.00878168510.4997067
CGTCTCA1000.008783638510.49939110
TAGGGTG1150.002076075610.434495
Produced by FastQC (version 0.11.2)