FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_15.35100000038a23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_15.35100000038a23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1692799
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCTC550.00210276316.36297610
CCGCCCG653.4388743E-416.1531949
TAGTCAG2550.015.2935019
GACTCGC750.001018605813.9994346
TAGGTAG3300.013.6358145
CAGGAGT1106.9168946E-613.6358144
GGTAGTC3350.012.984557
CGGTCCA850.002613934212.35244310
AGGGCGT1101.0438538E-412.2722326
CGATCAC1354.208814E-612.22281224
TAGGGCG900.00400963111.6661965
CCCCGGC1059.718224E-411.42810913
GTATTAG5450.011.2876161
AGTCAGA3600.011.24954610
CCTAGCG1355.6464087E-511.11197630
GGACTGC1501.3608915E-510.9995566
GGTACGC1408.181776E-510.7138538
TTAGGTA4950.010.6056334
TATTAGG6200.010.4028052
AGACTGC1451.1676318E-410.344416
Produced by FastQC (version 0.11.2)