FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_14.351000000389ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_14.351000000389ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549587
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCC600.003755701714.9994037
ACCCGGC600.003755701714.9994037
CTGTCCG600.00375650814.99891959
GTCCAGG902.649426E-413.3353841
GGTGAGC850.002613750512.3524488
TCTATAC4050.011.8513793
GCTATAG1355.6413235E-511.1128211
TAGACAG2053.205787E-810.9751735
AGCGCAC1100.001434033410.90830410
CCTAGAC1253.4240496E-410.799573
TGCGGGG1000.008784085510.49924412
CAGTGCG1000.008784085510.4992449
CGTCCTG1000.008784085510.4992449
CTAATAC4600.010.4343673
CTAGACC1756.616472E-610.2853064
GTCTTAT2505.180482E-910.2015691
TATTAGG4600.010.1082942
CCCTACA1356.9605897E-49.9996022
TAATACT6750.09.9996014
CCGTCCA1200.0029566169.9992799
Produced by FastQC (version 0.11.2)