FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n01_ntr_13.351000000389ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n01_ntr_13.351000000389ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1849271
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGT800.001657611613.12443210
CTGAGCG1507.393828E-812.9994379
GTATTAG4200.012.146931
CCTAGCG1252.5746274E-512.00045430
GAGGCCC1006.454587E-411.9994819
TATACTG7450.011.6773465
TCGGGGG900.00400995911.66616116
ACTGGCC2155.4151315E-911.1623088
GAGCGCA950.006000874611.0521559
ACCCTGC1501.361123E-510.9995248
ACCGCTC1100.001433915810.9086198
CGATCAC1807.8731864E-710.83403624
CTGGACC1807.884173E-710.8328644
ACTGAGC2402.6557245E-910.624548
GTATAGA5650.010.6230311
ACAGTCC1704.6826663E-610.5877768
GGCACGC1000.00878328810.49954612
TAGGGGG1000.00878328810.4995467
TCTGGAC2002.690049E-710.4995463
CCCTGCC1150.002076524510.4343319
Produced by FastQC (version 0.11.2)