FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_96.35200000039e50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_96.35200000039e50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707010
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT42770.6049419385864414No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC33780.4777867356897356No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC29450.41654290604093297No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC25930.36675577431719497No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC25670.3630783157239643No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG23890.3379018684318468No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG22310.3155542354422144No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC21180.2995714346331735No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT20610.2915093138710909No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19750.27934541237040494No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG19660.27807244593428665No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT18950.26803015516046447No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT18810.2660499851487249No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG14960.21159530982588648No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT14850.21003946195951967No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT14260.20169445976718856No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC14240.20141157833694004No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG13830.1956125090168456No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC13750.19448098329585153No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG13720.19405666115047876No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT13610.19250081328411195No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC13390.18938911755137833No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT12820.18132699678929576No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG12620.17849818248681065No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA12580.17793241962631362No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG11840.1674658067071187No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC11800.1669000438466217No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC11610.16421267025926084No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG11460.162091059532397No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT11380.16095953381140296No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA11200.15841360093916634No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC10360.14653258086872886No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC10100.14285512227549824No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC10060.1422893594150012No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG9910.14016774868813736No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC9800.13861190082177055No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT9630.13620740866465822No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT9580.13550020508903693No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT9510.13451012008316715No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT9470.13394435722267012No Hit
GTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTA9130.12913537290844543No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA9110.1288524914781969No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG9070.1282867286176999No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT9000.1272966436118301No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT8530.12064893000099008No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA8530.12064893000099008No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA8490.12008316714049307No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC8360.11824443784387774No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT8330.11782011569850498No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC8320.11767867498338072No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC8310.11753723426825646No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC8200.11598138640188965No Hit
GGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGA8010.11329401281452878No Hit
CCGTTATCCGGATCGAGGACAGTGTATGGTGGGTAG7880.11145528351791346No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC7840.11088952065741645No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG7750.10961655422129814No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC7730.10933367279104964No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA7720.10919223207592538No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT7710.10905079136080112No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC7350.10395892561632793No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT7320.10353460347095515No Hit
ATCCGGATCGAGGACAGTGTATGGTGGGTAGTTTGA7310.1033931627558309No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA7280.10296884061045813No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG7140.10098867059871854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTG3000.026.9991723
GTACCCG3200.025.3135151
ACTCGGT1600.025.3117263
CCCGTGC3400.023.8227984
TCGACTT3550.023.6629217
CTCGGTC1650.023.6356374
CGACTTG3650.023.0146218
ACCGTAT7950.022.8294877
GTGTTAG3550.022.81781628
GTTAGGC3550.022.81781630
TGTGTTA3550.022.81781627
CCGTGCT3550.022.8162025
TAAGTTA402.4655362E-422.50090226
TGTTAGG3600.022.50090229
AGTTAAC402.4655362E-422.50090228
CCACCGT8000.022.4993115
TCTATTA402.466717E-422.49931121
CTTGCAT3750.022.39931521
AGTCACA3300.022.27204312
GACTTGC3800.022.1061519
Produced by FastQC (version 0.11.2)