FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_95.35200000039e1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_95.35200000039e1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972543
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT76620.7878314891989351No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC53540.550515504198786No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC39920.4104702825479182No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC39430.40543194491143325No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG34850.35833891149285946No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG32060.32965123393001644No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC28490.292943345435626No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT27930.2871852452796432No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG27830.28615701310893193No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG27400.2817356147748737No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC27340.28111867547244695No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT26060.2679573036873434No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25470.26189073388014716No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT24970.2567495730265911No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG23730.243999494109772No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT22670.2331002331002331No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC22470.23104376875881066No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG22250.228781657983246No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA22000.2262110775564679No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC20610.211918650383582No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT20170.20739442883245265No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG20080.20646901987881253No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC20030.20595490379345693No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT19840.20400126266910562No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG19390.19937421790090518No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT19100.19639234460584262No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT18580.1910455373181443No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC18380.18898907297672185No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG17630.1812773316963877No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG17450.17942651378910754No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC17250.17737004944768509No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT15730.16174092045287458No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA15350.15783363820417196No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT15300.15731952211881634No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC14960.1538235327383982No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT14950.15372070952132708No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG14550.14960778083848222No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT14290.14693437719463304No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC14170.14570049858977957No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC13380.13757746444116095No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA13220.135932292968023No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC13170.1354181768826674No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC13150.13521253044852516No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC13110.13480123758024068No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC12860.1322306571534626No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA12680.13037983924618243No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA12560.12914596064132897No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT12550.12904313742425785No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG12370.12719231951697765No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG12340.1268838498657643No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT12270.12616408734626644No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA12230.12575279447798196No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT12100.12441609265605737No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA11740.12071445684149698No Hit
GGCTAGGCCAGCCTGGCGTTGGTTGTCCAGGTTTAA11200.11516200311965641No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC11180.11495635668551415No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC11140.11454506381722968No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA11060.11372247808066069No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT11050.11361965486358958No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC11030.11341400842944734No Hit
GGCCTAGCCTTCTCCGTCCCTCCATCGCAATAACCA10770.11074060478559818No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG10650.10950672618074472No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC10600.1089926100953891No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA10520.10817002435882012No Hit
TCTTTTGATGATGCGGTAGAGGAGCGTTCTGTAAGC10440.10734743862225114No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT10360.10652485288568218No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC10150.10436556532718862No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG10020.10302886350526404No Hit
CCCTATTAAGACTCGCTTTCGCTACGCCTCCCCTAT10010.10292604028819291No Hit
CTCCTCTACCGCATCATCAAAAGATGATACCCGTAG9810.10086957594677048No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC9750.10025263664434375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT254.2270037E-429.9986135
GTACCCG5200.026.827061
ACCCGTG5300.026.3195383
CCCGTGC5400.026.1099034
CCGTGCT5750.024.5206035
TATGCGC5600.024.37261811
CTTGCAT5800.023.79444921
TTGCATG5850.023.59107822
TGTGTTA5950.023.1945927
GTTAGGC6150.023.17204330
GTGTTAG6050.023.05915528
ACTCGGT2150.023.022193
CATGTGT6050.022.81120725
ACTTGCA6200.022.74322120
TGTTAGG6150.022.6842129
GTCTCTT603.2840762E-722.50127230
CCACCGT10550.022.3212415
ACCGTAT10500.022.2846817
GACTTGC6350.022.20483819
ATGTGTT6250.022.08238426
Produced by FastQC (version 0.11.2)