FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_94.35200000039dd7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_94.35200000039dd7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691651
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT54820.7925962660359054No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC27330.3951414803130481No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC23520.3400558952419645No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG20350.29422353181011807No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG19380.2801991177631494No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18220.26342765354203207No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG15620.22583644063263122No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT15520.2243906247515004No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT15500.22410146157527422No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG14870.2149928215241502No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC14600.21108911864509702No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA14600.21108911864509702No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT14590.21094453705698393No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC14570.21065537388075778No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG13800.1995225915960506No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG13770.19908884683171135No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT13720.19836593889114595No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG12170.17595579273361855No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT12030.17393165050003542No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC11570.16728089744683375No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT11170.16149763392231054No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC11150.16120847074608438No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG10970.15860600216004891No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC10930.1580276758075966No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC10830.1565818599264658No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC10690.15455771769288268No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG10300.14891903575647256No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT10200.14747321987534173No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT10050.14530449605364557No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA9420.1361958560025215No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC9200.13301506106403374No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC9090.13142466359478985No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT9030.13055717406611136No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG9020.1304125924779983No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT8990.12997884771365906No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT8900.12867761342064135No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA8880.12838845024441517No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC8780.12694263436328437No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG8450.12217144195555273No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA8390.12130395242687425No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC8290.11985813654574345No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA8230.11899064701706497No Hit
GGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTT8200.11855690225272572No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC7890.11407487302122024No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC7750.11205073078763711No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT7740.11190614919952403No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT7560.1093036806134886No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG7520.10872535426103627No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC7440.10756870155613164No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC7370.10655663043934006No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC7370.10655663043934006No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC7230.10453248820575696No Hit
CTTGTAGGTATTCGGAGTTTGCATCGGTTTGGTAAG7190.10395416185330464No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC7150.1033758355008523No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG7130.10308667232462615No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC7050.1019300196197215No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA7030.10164085644349535No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG7010.10135169326726919No Hit
ATGTATCACTGTAGGGGGTAGAGCACTGTTTCGGCT6970.10077336691481688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA300.0012264424.99867429
ACCCGTG1450.024.8262713
GTGTTAG1450.023.79184328
TGTGTTA1550.022.25688427
ACTCGGT1350.022.2210453
TGTTAGG1600.021.56135729
TTAGGCC350.003005882521.42743525
CTCGGTC1400.021.4274354
GTTAGGC1700.021.17534830
TGTAGGA859.622454E-1021.1753482
CCGTATG5550.021.0799648
CCCGTGC1800.020.8322284
ACCGTAT5700.020.5252277
CACCGTA5750.020.3467466
CTTGCAT1700.020.29597721
TCCGAAA608.247231E-619.998943
TAGGATA608.247231E-619.998944
CCGTGCT1800.019.9989385
CGTATGC5950.019.9149119
GTATGCG5950.019.66282510
Produced by FastQC (version 0.11.2)