HK5NHBGXX_l03n02_ntr_93.35200000039d94.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HK5NHBGXX_l03n02_ntr_93.35200000039d94.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77171
Sequences flagged as poor quality	0
Sequence length	36
%GC	56

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	466	0.6038537792694146	No Hit
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT	352	0.45612989335372095	No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC	305	0.3952261860025139	No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC	300	0.388747068199194	No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT	233	0.3019268896347073	No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT	195	0.2526855943294761	No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC	194	0.25138977076881214	No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG	180	0.2332482409195164	No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT	156	0.20214847546358086	No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT	140	0.1814152984929572	No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC	133	0.17234453356830934	No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT	133	0.17234453356830934	No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG	132	0.17104871000764535	No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG	125	0.16197794508299748	No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA	125	0.16197794508299748	No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC	122	0.15809047440100554	No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC	122	0.15809047440100554	No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG	114	0.14772388591569374	No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG	113	0.14642806235502975	No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG	111	0.14383641523370178	No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC	111	0.14383641523370178	No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG	109	0.14124476811237383	No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC	105	0.1360614738697179	No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT	104	0.1347656503090539	No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC	104	0.1347656503090539	No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC	103	0.13346982674838995	No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC	102	0.13217400318772596	No Hit
ACCAGGAACCGTACCCCAAACCGACACAGGTGGTTG	102	0.13217400318772596	No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC	99	0.12828653250573402	No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT	89	0.11532829689909423	No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA	87	0.11273664977776625	No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC	86	0.11144082621710229	No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC	85	0.1101450026564383	No Hit
GGTACGCAGTCACCCAACAAAGTGGGCTCCCACTGC	83	0.10755335553511035	No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG	83	0.10755335553511035	No Hit
CCCATTATACAAAAGGTACGCAGTCACCCAACAAAG	83	0.10755335553511035	No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT	82	0.10625753197444636	No Hit
GGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAG	81	0.10496170841378237	No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA	80	0.10366588485311841	No Hit
GGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC	80	0.10366588485311841	No Hit
GAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATAG	79	0.10237006129245442	No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC	78	0.10107423773179043	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTC	55	1.383687E-7	24.545454	27
TGGGGCG	50	1.6636859E-6	24.0	22
GGGGCGG	50	1.6636859E-6	24.0	23
GGTCTCC	60	3.205423E-7	22.5	28
GCGGTCT	55	3.807958E-6	21.818182	26
GTCTCCT	55	3.807958E-6	21.818182	29
TCTCCTC	55	3.807958E-6	21.818182	30
AGTCTTA	35	0.0029810972	21.428572	26
TGGCTTA	45	5.403351E-4	20.000002	21
CAGCTAG	40	0.0064568706	18.749998	9
GAGGCGT	40	0.0064568706	18.749998	9
GCTAGGA	40	0.0064568706	18.749998	11
AGCTAGG	40	0.0064568706	18.749998	10
ATAGGGG	40	0.0064568706	18.749998	3
GAGTCTT	40	0.0064568706	18.749998	25
GTCTTAA	40	0.0064568706	18.749998	27
TTGGCTT	40	0.0064568706	18.749998	20
GAATAGG	40	0.0064568706	18.749998	1
TAGGGGA	40	0.0064568706	18.749998	4
AAAGCGA	40	0.0064568706	18.749998	20

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content