FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_92.35200000039d51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_92.35200000039d51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141110
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT7680.5442562539862519No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC4430.3139394798384239No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC4330.3068528098646446No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3620.2565374530508114No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC3390.240238112111119No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG3230.2288994401530721No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3220.22819077315569414No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG2940.20834809722911204No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2920.2069307632343562No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2850.20197009425271065No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG2590.1835447523208844No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG2500.17716674934448304No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG2250.15945007441003473No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2220.15732407341790094No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG2200.15590673942314506No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC2190.15519807242576714No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG2100.14882006944936577No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC2030.14385940046772022No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC1960.1388987314860747No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1930.1367727304939409No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC1930.1367727304939409No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1890.1339380625044292No Hit
GGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC1840.1303947275175395No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1810.1282687265254057No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1780.12614272553327194No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1690.11976472255687053No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1670.11834738856211467No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1640.11622138756998088No Hit
TCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGG1630.11551272057260292No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC1560.11055205159095739No Hit
GTGATACATGAGGCGCTACCTAAATAGCTTTCGAGG1540.10913471759620155No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC1520.10771738360144567No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC1520.10771738360144567No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT1490.10559138260931189No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG1480.10488271561193395No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1480.10488271561193395No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1460.1034653816171781No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG1430.1013393806250443No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1430.1013393806250443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCTT200.005132443729.99964730
TAATAGC501.6802878E-623.99971617
CGTAATA501.6802878E-623.99971615
GCGTAAT501.6802878E-623.99971614
CCGTATG950.023.6839318
CACCGTA1050.022.8568736
ATTAAAC402.4528557E-422.4997353
ATGCGCA804.4201443E-1022.49973512
ACCGTAT1000.022.4997337
ATCCACC1150.022.173653
TATGCGC755.155016E-921.9997411
GTAATAG553.8456637E-621.81792316
CCACCGT1100.021.8179235
GGAGAAC350.00299345721.42831818
CTGAGAC350.00299345721.42831825
TGGAACT350.00299345721.42831820
GAGAACC350.00299345721.42831819
CATACTC350.00299345721.4283183
CTAGGTA706.013579E-821.4283185
CCACTTA859.422365E-1021.1762225
Produced by FastQC (version 0.11.2)