FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_91.35200000039d1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_91.35200000039d1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124215
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT11020.887171436621986No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC5300.4266795475586684No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC4800.38642676005313364No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3890.3131666867930604No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG3590.2890150142897395No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG3550.2857947912892968No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG3420.27532906653785777No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC3100.24956728253431548No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2870.23105100028176953No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2820.22702572153121603No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG2640.21253471802922352No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2580.20770438352855936No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2560.20609427202833794No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG2480.1996538260274524No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC2460.19804371452723102No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC2260.1819425995250171No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG2250.1811375437749064No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC2100.16906170752324598No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG2050.1650364287726925No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA2020.16262126152236042No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT2000.16101115002213903No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1900.15296059252103208No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1850.1489353137704786No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG1740.14007970051926097No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC1620.13041903151793263No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1600.12880892001771121No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT1600.12880892001771121No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1570.12639375276737916No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC1570.12639375276737916No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA1550.12478364126715774No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1530.12317352976693637No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC1520.12236847401682566No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1500.12075836251660428No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC1490.11995330676649357No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT1480.11914825101638289No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1450.11673308376605081No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC1440.1159280280159401No Hit
ATGTATCACTGTAGGGGGTAGAGCACTGTTTCGGCT1370.11029263776516524No Hit
ATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGTA1340.10787747051483315No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC1340.10787747051483315No Hit
GGCTAGGCCAGCCTGGCGTTGGTTGTCCAGGTTTAA1310.10546230326450105No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1310.10546230326450105No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT1310.10546230326450105No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA1260.1014370245139476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACA200.00512980530.0000024
TCTAGCC200.00512980530.00000220
CCGTCTA200.00512980530.00000217
GCACCTA254.2048845E-430.00000213
CCTCTTG254.2048845E-430.0000027
CCGTGCT300.001220403125.0000025
CCCGTGC300.001220403125.0000024
CGCTCGA300.001220403125.00000214
GCCATGA452.021708E-523.3333343
AGTCACA402.4503615E-422.50000212
ACTGAGA402.4503615E-422.50000224
TATGGTG957.2759576E-1222.1052634
GGTACTT350.00299125421.4285722
TCTTGCA350.00299125421.4285729
CTCTTGC350.00299125421.4285728
AGGAATT350.00299125421.42857215
CACCTAT350.00299125421.42857214
CGCCTCT350.00299125421.4285725
ACCTATG350.00299125421.42857215
GCCTCTT350.00299125421.4285726
Produced by FastQC (version 0.11.2)