FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_89.35200000039c88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_89.35200000039c88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730829
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22450.3071854017834541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGC1300.019.61489327
GGGCCCG1251.8189894E-1219.19951823
CCCGGGG1251.8189894E-1219.19951826
TAGGCTG400.00650922418.7495295
CGGCCCG1301.8189894E-1218.46233714
AGCCCGG1202.0736479E-1017.4995610
GCCCGGA1303.45608E-1117.30844116
GAGCCCG1303.45608E-1117.3072599
CCGGCCC1451.0913936E-1116.5524413
GCCCGGG1451.0913936E-1116.5513125
AGGAGCC1351.1041266E-915.5551657
CCCGGAG1451.8189894E-1015.51791417
GAGGGCC1451.8189894E-1015.51685221
CGGAGGG1503.0377123E-1015.0006519
CCCGGCC1605.0931703E-1115.00064912
GCCCGGC1658.185452E-1114.54508911
GGAGGGC1658.185452E-1114.54508920
CCGGAGG1554.965841E-1014.51675818
GGAGCCC1453.0322553E-914.4823958
TAGACTG851.6325086E-414.1172925
Produced by FastQC (version 0.11.2)