FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_88.35200000039c45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_88.35200000039c45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051155
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27680.2633293852952229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC703.1116746E-517.1430748
AGAGAGG1951.1641532E-912.3072626
GTGTGCC1006.4474734E-412.0001529
GTCTTAG1406.3015286E-611.7875461
ACAGTGC1151.5779668E-411.739288
CTAAGGC1602.2597833E-611.2501433
GCTCTAG950.00598909711.0543491
AGTATCC1653.2691696E-610.9092298
GTATAAA4050.010.742411
TACAGGG1000.00877541910.5001337
CTGGACT1000.00877541910.5001334
TATACGG2156.438495E-810.4652495
TCTTATA4350.010.3449592
GCTCTAT1602.7685017E-510.3141031
TGTGCCA1756.6058037E-610.28584510
GTCCTAT1200.002949700710.00155451
TATACTG2905.657057E-109.8277115
CAATACT2651.3462341E-89.6227644
CCTATAC1250.0041393219.60012153
CTCTTAT3152.5102054E-109.525291
Produced by FastQC (version 0.11.2)