FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n02_ntr_87.35200000039bf3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n02_ntr_87.35200000039bf3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254901
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29630.23611424327496752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTC1803.2923708E-1013.3323597
CTAAGCC1651.8633727E-812.72735826
ACCTATA1907.730705E-1012.6321671
TAGGACT954.1918835E-412.6301534
GTATTAG2800.012.3220011
CTAATAC3550.011.8296513
ATCACAC2553.6379788E-1211.76525329
GTCGATT900.00400962111.66581411
CTAAGCA1806.270784E-811.665354
GCCTCAC1303.8438837E-511.53853820
TTAGGCT1059.721061E-411.4272814
TAGACTG1059.721061E-411.4272815
TAGTCAG1459.339745E-611.3784799
TAGGTAG2155.4133125E-911.16153055
TATTAGG2701.2732926E-1111.11032
CCTAATA3550.010.9851142
CTGAGGC1653.2694916E-610.90959815
TAAGCAG2957.2759576E-1210.6767615
TAATACT5650.010.618274
GCAGATC2301.549779E-810.434028
Produced by FastQC (version 0.11.2)